Melissa Goellner Mitchum
Web site: Mitchum Lab Office address:
Division of Plant Sciences
371H Life Sciences Center Office phone:
(573) 882-6152 Fax:
The major focus of research in the Mitchum lab is the molecular basis of plant-nematode interactions with an emphasis on the interaction between the soybean cyst nematode (SCN; Heterodera glycines) and its host plant, soybean.
Sedentary endoparasitic nematodes, such as SCN, are the most economically important group of plant-parasitic nematodes. SCN is consistently the most damaging pest of soybeans grown in Missouri and throughout the US, causing nearly 1 billion in crop losses annually.
After penetrating and migrating through soybean root tissue, SCN induces dramatic modifications of selected cells near the vasculature of the root to form an elaborate feeding cell (called a syncytium). Growth and development of the nematode is completely dependent on the formation of the syncytium from which it derives nutrients. We are studying the signal exchange that occurs between the nematode and its host for the formation of feeding cells. In addition to soybean, we use the Arabidopsis-beet cyst nematode pathosystem as a model system to dissect the mechanisms of pathogenesis and feeding cell formation. The aim of this research is to advance our understanding of the molecular basis of pathogenicity and host resistance to cyst nematodes with the long term goal of developing improved disease resistance strategies.
- *Brown S, *Yeckel G, *Heinz R, Clark K, Sleper D, and Mitchum MG. A high-throughput automated technique for counting females of Heterodera glycines using a fluorescence-based imaging system. Journal of Nematology 2011;(in press). *equal contributors
*Kandoth PK, *Ithal N, Recknor J, Maier T, Nettleton D, Baum TJ, and Mitchum MG. The soybean Rhg1 locus for resistance to the soybean cyst nematode Heterodera glycines regulates expression of a large number of stress- and defense-related genes in degenerating feeding cells. Plant Physiology 2011; doi 10.1104/pp.110.167536. *equal contributors
*Lee C, *Chronis D, Kenning C, Peret B, Hewezi T, Davis EL, Baum TJ, Hussey RS, Bennett M and Mitchum MG. The novel cyst nematode effector protein 19C07 interacts with the Arabidopsis auxin influx transporter LAX3 to control feeding site development. Plant Physiology 2011;155:866-880. *equal contributors
Replogle A, Wang J, Bleckmann A, Hussey RS, Baum TJ, Shinichiro S, Davis EL, Wang X, Simon R, and Mitchum MG. Nematode CLE signaling in Arabidopsis requires CLAVATA2 and CORYNE. Plant Journal 2011;65(3):430-440.
Wang J, Replogle A, Hussey R, Baum T, Wang X, Davis EL, and Mitchum MG. Identification of potential host plant mimics of CLV3/ESR (CLE)-like peptides from the plant-parasitic nematode Heterodera schachtii. Molecular Plant Pathology 2011;12(2):177-186.
Wang J, Joshi S, Korkin D, and Mitchum MG. Variable domain I of nematode CLEs directs post-translational targeting of CLE peptides to the extracellular space. Plant Signaling and Behavior 2010;5(12):1-3.
Wang J, Lee C, Replogle A, Joshi S, Korkin D, Hussey R, Baum TJ, Davis EL, Wang X and Mitchum MG. Dual roles for the variable domain in protein trafficking and host-specific recognition of Heterodera glycines CLE effector proteins. New Phytologist 2010;187(4):1003-1017.
Patel N, Hamamouch N, Li C, Hewezi T, Hussey RS, Baum TJ, Mitchum MG and Davis EL. A nematode effector protein similar to annexins in host plants. Journal of Experimental Botany 2010;61(1):235-248.
Hewezi T, Howe PJ, Maier TR, Hussey RS, Mitchum MG, Davis EL and Baum TJ. Arabidopsis spermidine synthase is targeted by an effector protein of the cyst nematode Heterodera schachtii. Plant Physiology 2010;152(2):968-984.